Adding sodium chloride into a medium containing 140g·L-1 glucose could promote the glycerol accumulation efficiently

but the glycerol productivity would decrease when sodium chloride concentration was above 40g·L-1.Experimental results in shake flask showed that sodium chloride concentration of 40g·L-1 was favorable.Moreover

the feasibility of multi-stage cascade continuous fermentation was tested and the results indicated that multi-stage continuous system was superior to single-stage continuous fermentation.Compared with batch fermentation

the glycerol concentration and the glycerol yield in multi-stage cascade continuous system decreased by only 9.83 % and 5.71 % respectively

but the glycerol productivity increased by 18.54% even the preparing time between two batch fermentation was not considered.