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1. 天津大学化工学院化学工程研究所
2. 吉林化工学院应用与制药系
Online First:25 December 2002,
Published:25 December 2002
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CAO Hongbin, JIANG Bin, LI Xingang, et al. NOVEL METHOD EXTRACTING EXTRACELLUAR POLYMERIC SUBSTANCES FROM INTACT BIOFILMS[J]. CIESC Journal, 2002, (12).
DOI:
CAO Hongbin, JIANG Bin, LI Xingang, et al. NOVEL METHOD EXTRACTING EXTRACELLUAR POLYMERIC SUBSTANCES FROM INTACT BIOFILMS[J]. CIESC Journal, 2002, (12). DOI:
It is very important to find a effective method to extract the extracelluar polymeric substances (EPS) including polysaccharides
proteins and DNA
which have a significant role in the metabolism of the cells and the mass transfer of substrate within the biofilms
from biofilms without destroying its structure. In this study
a novel method to extract the EPS from intact biofilm was set up
based on the enhancement of external alternating electric field. The results showed that the current had insignificant effect on the cells structure
but it could enhance the extraction rate when charged extractant is used. In the range from 10 Hz to 500 Hz
the extraction rate increased with current frequency.
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