OXIDATION AND ADSORPTION OF CATECHOL WITH POLYPHENOL OXIDASE-CELITE COUPLED SYSTEM IN AQUEOUS MEDIA

LIU Lin; SHI Zhengce; WANG Quan; JIN Jiangnan; FAN Yun

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OXIDATION AND ADSORPTION OF CATECHOL WITH POLYPHENOL OXIDASE-CELITE COUPLED SYSTEM IN AQUEOUS MEDIA

更新时间：2025-12-10

- OXIDATION AND ADSORPTION OF CATECHOL WITH POLYPHENOL OXIDASE-CELITE COUPLED SYSTEM IN AQUEOUS MEDIA
- CIESC Journal Issue 2, Pages: 221-225(2003)
- 作者机构：
  
  浙江工业大学生物与环境工程学院
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Online First：25 February 2003，
  
  Published：25 February 2003
- 稿件说明：
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LIU Lin, SHI Zhengce, WANG Quan, et al. OXIDATION AND ADSORPTION OF CATECHOL WITH POLYPHENOL OXIDASE-CELITE COUPLED SYSTEM IN AQUEOUS MEDIA[J]. CIESC Journal, 2003, (2): 221-225.
DOI：

LIU Lin, SHI Zhengce, WANG Quan, et al. OXIDATION AND ADSORPTION OF CATECHOL WITH POLYPHENOL OXIDASE-CELITE COUPLED SYSTEM IN AQUEOUS MEDIA[J]. CIESC Journal, 2003, (2): 221-225. DOI：

摘要

研究了多酚氧化酶-硅藻土耦合系统在苯甲醚共存条件下氧化邻苯二酚及产物吸附过程的特性.多酚氧化酶表现出对酚类物质的特异性催化作用，不受共存非酚类物质的影响；硅藻土与反应产物之间的特异性吸附作用有利于从溶液中分离邻苯二酚.耦合系统对酶促反应具有增强效应，多酚氧化酶在溶液中的相应半衰期增加2.4倍；当硅藻土/酶的质量比为6时，最大分离效率达到98.76 %.

Abstract

The oxidation/adsorption properties of polyphenol oxidase（PPO）-celite coupled system in the separation of catechol from aqueous media containing anisole were studied.The enzyme showed exclusive substrate selectivity for catechol oxidation.Celite adsorbed the enzyme-generated products subsequently.The coexisting anisole presented no undesirable influence on the reaction.This phenomenon indicated that the coupled system enhanced the elimiation process of catechol.PPO can be easily separated from aqueous media.The half-life of the enzyme increased about 2.4 folds and the corresponding adsorption efficience could be as high as 98.76 %.

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