CELLULASE ACTIVITIES IN AQUEOUS SOLUTIONS IN PRESSURIZED CARBON DIOXIDE

HE Weizhong; YAO Shanjing; GUAN Yixin; ZHU Ziqiang

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CELLULASE ACTIVITIES IN AQUEOUS SOLUTIONS IN PRESSURIZED CARBON DIOXIDE

更新时间：2025-12-10

- CELLULASE ACTIVITIES IN AQUEOUS SOLUTIONS IN PRESSURIZED CARBON DIOXIDE
- CIESC Journal Issue 12, (2002)
- 作者机构：
  
  浙江大学化学工程与生物工程学系
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Online First：25 December 2002，
  
  Published：25 December 2002
- 稿件说明：
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HE Weizhong, YAO Shanjing, GUAN Yixin, et al. CELLULASE ACTIVITIES IN AQUEOUS SOLUTIONS IN PRESSURIZED CARBON DIOXIDE[J]. CIESC Journal, 2002, (12).
DOI：

HE Weizhong, YAO Shanjing, GUAN Yixin, et al. CELLULASE ACTIVITIES IN AQUEOUS SOLUTIONS IN PRESSURIZED CARBON DIOXIDE[J]. CIESC Journal, 2002, (12). DOI：

摘要

测定了10％-40％(质量)乙醇-水溶液中的纤维素酶在温度为20-40℃和CO2压力为0.49-4.41MPa下的酶活变化数据

这些数据是用高压CO2分离纯化纤维素酶的基础数据.实验表明

CO2压力不是酶活损失的主要因素

乙醇浓度和温度对酶活损失的影响较大.在乙醇浓度小于30％、温度低于35℃的条件下

对纤维素酶-乙醇-水体系进行高压CO2处理比较适宜.

Abstract

The activities of cellulase in 10%-40% ethanol- water solution were investigated at temperatures of 20-40 ℃ and at pressures of 0.49-4.41 MPa. These data obtained are essential to purify cellulase by high pressure CO2. The experimental results indicate that CO2 pressure is not the key factor of the loss of enzyme activities

but ethanol concentration and temperature markedly influence recovered activities. The optimum operating conditions are 30 % ethanol concentration solution and 35 ℃ for cellulase- ethanol- water systems using high pressure CO2.

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