In this work

the effect of alcohols (including alcohols containing single

two

three hydroxyl groups

respectively) on the enzyme- catalyzed synthesis of Z-L-Asp-PheOMe (ZAPM) is investigated by using a new technique of enzymatic catalysis in a heterogeneous pseudo-eutectic solid-to-solid mixture with thermolysin.The experimental result shows that the alcohols containing two hydroxyl groups are the most suitable adjuncts for the studied enzymatic synthesis system

and the yield of ZAPM for 1

4-butanediol as an adjunct reaches 95% at reaction time 2h

which is better than the results in two-phase systems and other pseudo-eutectic solid-to-solid mixture reported in literature.