KINETICS OF LYSOZYME REFOLDING FACILITATED BY MOLECULAR CHAPERONE GroEL

DONG Xiaoyan; BAI Shu; LIU Xiaoguang; SUN Yan

您当前的位置：

首页 >

文章列表页 >

KINETICS OF LYSOZYME REFOLDING FACILITATED BY MOLECULAR CHAPERONE GroEL

更新时间：2025-12-10

- KINETICS OF LYSOZYME REFOLDING FACILITATED BY MOLECULAR CHAPERONE GroEL
- CIESC Journal Issue 12, (2001)
- 作者机构：
  
  天津大学化工学院生物工程系
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Online First：25 December 2001，
  
  Published：25 December 2001
- 稿件说明：
移动端阅览
DONG Xiaoyan, BAI Shu, LIU Xiaoguang, et al. KINETICS OF LYSOZYME REFOLDING FACILITATED BY MOLECULAR CHAPERONE GroEL[J]. CIESC Journal, 2001, (12).
DOI：

DONG Xiaoyan, BAI Shu, LIU Xiaoguang, et al. KINETICS OF LYSOZYME REFOLDING FACILITATED BY MOLECULAR CHAPERONE GroEL[J]. CIESC Journal, 2001, (12). DOI：

摘要

建立了溶菌酶复性的表观动力学模型

研究了分子伴侣GroEL促进变性溶菌酶复性的动力学行为

包括酶浓度、三磷酸腺苷 (ATP)浓度、GroEL与酶的摩尔比对复性率和复性速率常数的影响 .酶浓度对复性速率常数的影响比对复性率的影响显著 ;酶的复性率和复性速率常数随ATP浓度的增大而提高 ;在蛋白质浓度一定的情况下

存在适宜的GroEL和ATP浓度

使复性率和复性速率常数最大

Abstract

An apparent kinetic model for lysozyme refolding was established

and the refolding behavior of denatured lysozyme facilitated by the molecular chaperone GroEL was studied.The effects of denatured lysozyme concentration

adenosine triphosphate (ATP) concentration

and the molar ratio of GroEL to lysozyme on the refolding yield and the refolding rate constant were investigated.The lysozyme concentration showed more significant effect on the refolding rate constant than on the refolding yield.Both refolding yield and refolding rate constant increased with increasing ATP concentration.At a definite protein concentration

optimal concentrations of both GroEL and ATP existed to give maximum refolding yield and refolding rate constant.

关键词

Keywords

references

Views

143

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Identification of gas-liquid two-phase flow patterns in a riser based on recursive characteristics and CNN

Mixed matrix membranes based on nitrogen-rich Schiff base network polymer and their CO2 separation performance

Performance of alkali metal-loaded SrMnO3 oxygen carriers in chemical looping oxidative dehydrogenation of ethylbenzene

Heat transfer mechanisms, bottleneck identification and optimization in heat exchanger networks under variable operating conditions

Engineering verification and NO emission control of rare earth Y2O3-based catalytic coatings on a metal fiber surface burner

Related Author

No data

Related Institution

No data

⁰