INFLUENCE OF MIXING DEVICE ON SERUM-FREE CULTIVATION OF INSECT CELLS IN SPINNER FLASKS

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INFLUENCE OF MIXING DEVICE ON SERUM-FREE CULTIVATION OF INSECT CELLS IN SPINNER FLASKS

更新时间：2025-12-16

- INFLUENCE OF MIXING DEVICE ON SERUM-FREE CULTIVATION OF INSECT CELLS IN SPINNER FLASKS
- CIESC Journal Issue S1, (2000)
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  CLC： 962
- Online First：30 December 2000，
  
  Published：30 December 2000
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INFLUENCE OF MIXING DEVICE ON SERUM-FREE CULTIVATION OF INSECT CELLS IN SPINNER FLASKS[J]. CIESC Journal, 2000, (S1).
DOI：

INFLUENCE OF MIXING DEVICE ON SERUM-FREE CULTIVATION OF INSECT CELLS IN SPINNER FLASKS[J]. CIESC Journal, 2000, (S1). DOI：

摘要

Abstract

A culture system was developed and successfully employed for the serum-free cultivation of Tn-5B1-4 (Tn5 ) insect cells. With our adaptation procedure

it was possible to obtain cells fully adapted to serum-free media in stationary T-flasks and then enable these adapted cells to grow well in spinner flasks immediately. The spinner "ask with special stirring design proved tO provide favorable culture environment that made it desirable for use in the serum-free cultivation of Tn5 cells even at low seeding density.

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