CIESC Journal ›› 2020, Vol. 71 ›› Issue (10): 4711-4719.doi: 10.11949/0438-1157.20200346

• Separation engineering • Previous Articles     Next Articles

Magnetic temperature-sensitive molecularly imprinted materials for separation and enrichment of single component of formononetin in medicinal plants

Jiayuan HE1(),Zhuangfei JIANG1,Rongrong MA1,Lili YANG1,Qingyao LI1,Ling TAN2,Zhitao CHEN1,Qihui ZHANG1()   

  1. 1.School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400044, China
    2.School of Pharmacy, Chongqing University, Chongqing 400044, China
  • Received:2020-04-01 Revised:2020-05-22 Online:2020-10-05 Published:2020-07-07
  • Contact: Qihui ZHANG;


The magnetic and temperature double response molecularly imprinted polymers for formononetin-specific adsorption (MTMIPs) were successfully prepared by using Fe3O4 as supporting matrix, formononetin as template, N-isopropyl acrylamide as the thermo-sensitive type functional monomer and methacrylic acid as auxiliary functional monomer. SEM, TEM, FT-IR, TGA and magnetic analysis were used to characterize the structure of MTMIPs, and then their adsorption properties and reproducibility were investigated. The results showed that MTMIPs was a core-shell structure with good thermal stability, good adsorption performance (16.43 mg/g) and fast adsorption performance. The adsorption kinetics of formononetin was consistent with the quasi-second-order kinetics model, and the adsorption process was consistent with the Langmuir monolayer adsorption with good reproducibility. HPLC test results show that MTMIPs can be used to separate and enrich formononetin from complex samples.

Key words: magnetic temperature-sensitive materials, molecular imprinted materials, polymers, adsorbents, nanomaterials

CLC Number: 

  • R 917


Schematic diagram of the synthesis process of magnetic temperature-sensitive MTMIPs"


SEM images of Fe3O4@SiO2NPs (a), MTMIPs (b), and TEM of MTMIPs (c)"


FT-IR spectra of MTMIPs and Fe3O4@SiO2 (a); TGA analysis curves of MTMIPs (b); Magnetization curves of MTMIPs and Fe3O4 nanoparticles (c); XRD pattern of Fe3O4, Fe3O4@SiO2 and MTMIPs nanoparticles(d)"


Temperature-sensitive adsorption spectra of MTMIPs"

Table 1

The fitting parameters of isothermal adsorption model"


Table 2

The fitting parameters of adsorption kinetics model"



HPLC diagrams of MTMIPs selectivity study (a); Selection adsorption of formononetin, genistein and daidzein by MTMIPs and MTNIPs (b)"

Table 3

Data of methodological examination in selective research"

SamplesRegression analysisPrecision
Standard curvesCorrelation coefficientLinear range/(μg/ml)LOD/(μg/ml)LOQ/(μg/ml)Intra-day RSD/%Inter-day RSD/%


HPLC diagrams of the MTMIPs practical application: (1) HPLC diagram of the formononetin mother solution; (2) The HPLC diagram of MTMIPs eluents; (3) HPLC diagram of crude extracts after MTMIPs absorbed; (4) HPLC diagram of crude extracts from Trifolium pratense L."

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