Abstract: The characteristics of granular sludge in the EGSB reactor treating TCM wastewater were analyzed in terms of particle size distribution (PSD), integrity coefficient (IC), metal content and extracellular polymeric substances (EPS) of granular sludge. When COD concentration was 2000-5000 mg·L^-1, hydraulic residence time (HRT) was 12 h, temperature was 30℃, the peak of PSD was in the range of 500-1000 μm, IC value was less than 20, and the contents of total EPS, protein, polysaccharide in granular sludge were 85.59, 63.67 and 21.92 mg·(g VSS)^-1, respectively. At this stage, flocculation of granular sludge was good, and its mechanical strength was high. When HRT was reduced to 6 h, IC value increased to 30.03, and the ratio of protein and polysaccharide increased to 6.86. However，the concentration of polysaccharide in granular sludge decreased to 18.11 mg·(g VSS)^-1. When temperature decreased to 20℃, the peak of PSD was in the range of 250-750 μm and IC value was 32.11. The concentrations of Ca²⁺, Mg²⁺ and Mn²⁺ were only 20.78, 4.79 and 0.94 mg·L^-1, respectively. At the same time, the contents of total EPS, protein, polysaccharide in granular sludge were 69.04, 58.87 and 10.17 mg·(g VSS)^-1, respectively. During this period, granular sludge was washed out from the EGSB reactor and COD removal efficiency decreased.

Key words: wastewater, anaerobic, EGSB reactor, granular sludge, particle size distribution, extracellular polymeric substances

摘要： 通过分析厌氧颗粒污泥粒径分布、机械强度、二价金属含量、胞外聚合物含量等，探讨了EGSB反应器处理中药废水过程中颗粒污泥特性变化。结果表明，在中药废水COD浓度为2000～5000 mg·L^-1、HRT为12 h、T为30℃的条件下，颗粒污泥粒径分布在500～1000 μm之间，完整系数（integrity coefficient，IC）小于20，颗粒污泥EPS总量、蛋白含量、多糖含量分别为85.59、63.67和21.92 mg·（g VSS）^-1，此时颗粒污泥絮凝性良好，机械强度高。当HRT减少为6 h时，IC为30.03，蛋白与多糖的比值增大到6.86，但多糖含量仅为18.11 mg·（g VSS）^-1；而当T降低为20℃时，颗粒污泥粒径分布在250～750 μm之间，IC增大到32.11，Ca²⁺、Mg²⁺、Mn²⁺含量减少 为20.78、4.79和0.94 mg·L^-1，颗粒污泥EPS显著降低，其总量、蛋白含量、多糖含量仅为69.04、58.87和10.17 mg·（g VSS）^-1，在此运行条件下，出现了颗粒污泥的解体、流失，同时出水水质变差。

关键词: 废水, 厌氧, EGSB反应器, 颗粒污泥, 粒径分布, 胞外聚合物