CIESC Journal ›› 2014, Vol. 65 ›› Issue (3): 1062-1067.DOI: 10.3969/j.issn.0438-1157.2014.03.041

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Effect of Cu2+ on biofilm and extracellular polymeric substance

HU Xuewei1, LI Shu1, RONG Ye2, JIANG Meng1, ZHANG Yan1, LI Yuan1   

  1. 1 Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming 650500, Yunnan, China;
    2 Faculty of Modern Agricultural Engineering, Kunming University of Science and Technology, Kunming 650500, Yunnan, China
  • Received:2013-06-19 Revised:2013-10-31 Online:2014-03-05 Published:2014-03-05
  • Supported by:

    supported by the National Natural Science Foundation of China(50908109,51178208, 51368024),the Key Projects in Yunnan Province Department of Education(2013Z123), the Kunming University of Science and Technology Analysis Test Fund(20140549) and the University-Enterprise Cooperation Projects(2013YT02).

Cu2+对生物膜及其胞外聚合物的影响

胡学伟1, 李姝1, 荣烨2, 江孟1, 张燕1, 李媛1   

  1. 1 昆明理工大学环境科学与工程学院, 云南 昆明 650500;
    2 昆明理工大学现代农业工程学院, 云南 昆明 650500
  • 通讯作者: 胡学伟
  • 作者简介:胡学伟(1979—),男,博士,副教授。
  • 基金资助:

    国家自然科学基金项目(50908109,51178208, 51368024);云南省教育厅重点项目(2013Z123);昆明理工大学分析测试基金(20140549);云铜校企业合作项目(2013YT02)。

Abstract: Through adding different concentration of Cu2+ in the biofilm reactor, the relationship between extracellular polymeric substance(EPS) secreted by biofilm and efficiency of copper removal by biofilm was studied. Biofilm tolerance of Cu2+ was better than activated sludge, but a high concentration of Cu2+ rapidly poisoned the biofilm. When Cu2+ < 2 mg·L-1, Cu2+ inhibited biofilm secretion of EPS. Concentration of 2 mg·L-1 < Cu2+ < 5 mg·L-1, Cu2+ would increase the amount of EPS, while Cu2+ > 5 mg·L-1, the biofilm system displayed serious instability. Comparing proteins/polysaccharide (PN/PS) value showed that the biofilm suffered inhibition by Cu2+. The biofilm would inhibit more extracellular polysaccharide secretion, and the biofilm resistance of Cu2+ indicated that the biofilm would secrete more extracellular protein. The amount of EPS had a good correlation with the enrichment ratio of Cu2+.

Key words: biofilm, copper stress, reactivity, extracellular proteins, extracellular polysaccharide

摘要: 利用自行设计的生物膜培养装置进行挂膜,通过向生物膜反应器中投加不同浓度的Cu2+,探讨生物膜上的胞外聚合物(extracellular polymeric substance, EPS)与生物膜去除金属铜之间的关系。研究结果表明:生物膜法较活性污泥法对Cu2+具有更好的耐受性;当Cu2+ < 2 mg·L-1,Cu2+会抑制生物膜分泌EPS,在2 mg·L-1 < Cu2+ < 5 mg·L-1时,生物膜分泌EPS的量增加,当Cu2+ > 5 mg·L-1,生物膜系统表现为严重的不稳定性;比较蛋白质(proteins,PN)/多糖(polysaccharide,PS)值发现,Cu2+对生物膜的抑制主要是对生物膜上胞外多糖的抑制,而生物膜对Cu2+的抵抗表现为分泌更多的胞外蛋白;EPS与Cu2+的富集率关系呈线性正相关。

关键词: 生物膜, 铜胁迫, 活性, 胞外蛋白, 胞外多糖

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