CIESC Journal ›› 2012, Vol. 63 ›› Issue (4): 1182-1188.DOI: 10.3969/j.issn.0438-1157.2012.04.027

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Metabolome analysis of industrial pleuromutilin fermentation process of Pleurotus mutilus

YANG Yang1, LI Xia1, HAN Fenghua2, HU Jianglin2, YUAN Yingjin1   

  1. 1Key Laboratory of Systems Bioengineering,Ministry of Education,Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China; 2Shandong Shengli Co., Ltd., Jinan 250101, Shandong, China
  • Received:2011-08-26 Revised:2011-12-05 Online:2012-04-05 Published:2012-04-05

截短侧耳素生产菌Pleurotus mutilus工业发酵过程代谢组分析

杨扬1,李霞1,韩风华2,胡江林2,元英进1   

  1. 1系统生物工程教育部重点实验室,天津大学化工学院制药工程系,天津 300072; 2山东胜利股份有限公司,山东 济南 250101
  • 通讯作者: 元英进

Abstract: Taking the industrial pleuromutilin fermentation with strain Pleurotus mutilus as an example, the variations of small molecule metabolomes in different industrial fermentation processes were analyzed.The small molecule metabolomes in different processes were measured by gas chromatography coupled to time-of-flight mass spectrometry.Principal component analysis(PCA)was performed on the metabolome of P.mutilus.According to the score plot and loading plot obtained by PCA, the similarities and differences of metabolism of P.mutilus in two processes were extracted and classified.The analysis on characteristic metabolites indicated significant effects of different processes on cell viabilities.The higher level of TCA cycle intermediates and amino acids during the seed culture stage in the process two indicated higher carbon and nitrogen metabolism in P.mutilus before the inoculation.In addition, the analysis of the variations of metabolites during the two different fermentation processes suggested that nutrition utilization efficiency of P.mutilus in process two was higher than that in process one.This study indicates that metabolome analysis could be a new approach to evaluating the industrial bioprocess and is helpful in providing the feasible regulation scheme to improve the fermentation process.

Key words: industrial process, metabolomics, principal components analysis, characteristic metabolites, pleuromutilin

关键词: 工业过程, 代谢组学, 主成分分析, 特征代谢物, 截短侧耳素

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