CIESC Journal ›› 2018, Vol. 69 ›› Issue (8): 3577-3584.doi: 10.11949/j.issn.0438-1157.20180054

Previous Articles     Next Articles

Bioactive coating prepared by bio-3D printing of castor oil-based waterborne polyurethane mixed with carbonic anhydrase

CUI Jiandong1,2, CUI Zhaohui1,2, SU Zhiguo2, ZHENG Chunyang3, MA Guanghui2, ZHANG Songping2   

  1. 1 College of Bioscience and Bioengineering, Hebei University of Science and Technology, Shijiazhuang 050018, Hebei, China;
    2 State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China;
    3 Beijing Organoid Bio-Tech Co., Ltd., Beijing 100192, China
  • Received:2018-01-15 Revised:2018-04-02 Online:2018-08-05 Published:2018-04-13
  • Supported by:

    supported by the National Natural Science Foundation of China (21676276, 21676069, 91534126).

Abstract:

Castor oil-based anionic waterborne polyurethane (WPU) mixed with carbonic anhydrase (CA) was used to prepare bioactive coatings by bio-3D printing technique and regular coating methods. Interactions between CA and WPU were studied by dynamic light scattering, IR, SEM-EDC, TGA, and contact angle analyses. The results showed that the CA could attach tightly to WPU nanoparticles through the electrostatic interactions with anionic groups on the polyurethane. The activity recovery of bioactive coatings prepared by bio-3D printing technology was 4-fold higher than that of the conventional coating method, reaching 50.51%. The higher activity recovery could be attributed to the thin layer, smooth surface, and uniform distribution of CA in the 3D printed coating.

Key words: bio-3D printing, castor oil based waterborne polyurethane, coating, enzyme, immobilization, biocatalysis

CLC Number: 

  • O643.3

[1] ZHANG X Y, ZHANG Y D. Tissue engineering applications of three-dimensional bioprinting[J]. Cell Biochem. Biophys., 2015, 72:777-782.
[2] 顾奇, 郝捷, 陆阳杰, 等. 生物三维打印的研究进展[J]. 中国科学:生命科学, 2015, 45(5):439-449. GU Q, HAO J, LU Y J, et al. Three-dimensional bio-printing[J]. Sci. China Life Sci., 2015, 45(5):439-449.
[3] DIASA A A, CARDOSO T M G, CARDOSO R M, et al. Paper-based enzymatic reactors for batch injection analysis of glucose on 3D printed cell coupled with amperometric detection[J]. Sensors and Actuators B:Chemical, 2016, 226:196-203.
[4] FISCHER C, FRAIWAN A, CHOI S. A 3D paper-based enzymatic fuel cell for self-powered, low-cost glucose monitoring[J]. Biosensors and Bioelectronics, 2016, 79:193-197.
[5] MANDRYCKY C, WANG Z J, KIM K Y, et al. 3D bioprinting for engineering complex tissues[J]. Biotechnology Advances, 2016, 34:422-434.
[6] GUPTA M K, MENG F, JOHNSON B N, et al. 3D printed programmable release capsules[J]. Nano Letter, 2015, 15(8):5321-5329.
[7] KAZENWADEL F, BIEGERT E, WOHLGEMUTH J. A 3D-printed modular reactor setup including temperature and pH control for the compartmentalized implementation of enzyme cascades[J]. Eng. Life Sci., 2016, 16:560-567.
[8] HOSSAIN S M Z, LUCKHAM R E, SMITH A M, et al. Development of a bioactive paper sensor for detection of neurotoxins using piezoelectric inkjet printing of sol-gel-derived bioinks[J]. Anal. Chem., 2009, 81:5474-5483.
[9] PETRI I, ANNI M, NIKLAS S. Printing technologies for biomolecule and cell-based applications[J]. International Journal of Pharmaceutics, 2015, 494:585-592.
[10] MORE A S, LEBARBE T, MAISONNEUVE L, et al. Novel fatty acid based di-isocyanates towards the synthesis of thermoplastic polyurethanes[J]. Eur. Polym. J., 2013, 49:823-833.
[11] ALAGI P, HONG S C. Vegetable oil-based polyols for sustainable polyurethanes[J]. Macromol. Res., 2015, 23(12):1079-1086.
[12] AKINDOYO J O, BEG M D H, GHAZALI S, et al. Polyurethane types, synthesis and applications -a review[J]. RSC Adv., 2016, 6:114453-114482.
[13] UHRICH D, LQNGERMANN J V. Preparation and characterization of enzyme compartments in UV-cured polyurethane-based materials and their application in enzymatic reactions[J]. Frontiers in Microbiology, 2017, 8:1-9.
[14] 徐雪, 钟尚富, 朱延安, 等. 新型TMPLA水性聚氨酯分散体的合成及助成膜性能[J]. 化工学报, 2016, 67(5):2124-2130. XU X, ZHONG S F, ZHU Y A, et al. Synthesis and film-forming performance of novel polyurethane aqueous dispersions[J]. CIESC Journal, 2016, 67(5):2124-2130.
[15] PFISTER D P, XIA Y, LAROCK R C. Recent advances in vegetable oil-based polyurethanes[J]. Chem. Sus. Chem., 2011, 4:703-717
[16] LU Y S, LAROCK R C. Soybean oil-based, aqueous cationic polyurethane dispersions:synthesis and properties[J]. Progress in Organic Coatings, 2010, 69:31-37.
[17] GURUNATHAN T, CHUNG J S. Physicochemical properties of amino-silane-terminated vegetable oil-based waterborne polyurethane nanocomposites[J]. ACS Sustainable Chem. Eng., 2016, 4:4645-4653.
[18] MADBOULY S A, XIA Y, KESSLER M R. Rheological behavior of environmentally friendly castor oil-based waterborne polyurethane dispersions[J]. Macromolecules, 2013, 46:4606-4616.
[19] BORJA F A, ARANTXA E. Salting-out waterborne catiomeric polyurethanes for drugs encapsulation and delivery[J]. Macromol. Chem. Phys., 2015, 216:1914-1924.
[20] LIU K, ZHANAG S P, SU Z G, et al. Preparation and characterization of castor oil-based cationic waterborne polyurethane[J]. Advanced Materials Research, 2015, 1090(3):3-7.
[21] OU C W, SU C H, JENG U S, et al. Characterization of biodegradable polyurethane nanoparticles and thermally induced self-assembly in water dispersion[J]. ACS Appl. Mater Interfaces, 2014, 6:5685-5694.
[22] GURUNATHAN T, ARUKULAB R, CHUNG J S, et al. Development of environmental friendly castor oil-based waterborne polyurethane dispersions with polyaniline[J]. Polym. Adv. Technol., 2016, 27:1535-1540.
[23] LIU K, SU Z G, MIAO S D, et al. Enzymatic waterborne polyurethane towards a robust and environmentally friendly antibiofouling coating[J]. RSC Adv., 2016, 6:31698-31704.
[24] LIU K, SU Z G, MIAO S D, et al. UV-curable enzymatic antibacterial waterborne polyurethane coating[J]. Biochemical Engineering Journal, 2016, 113:107-113.
[25] ZHANG L T, WU S T, BUTHE A, et al. Poly(ethylene glycol) conjugated enzyme with enhanced hydrophobic compatibility for self-cleaning coatings[J]. ACS Applied Materials and Interfaces, 2012, 4(7):5981-5987.
[26] PATEL T N, PARK A H A, BANTA S. Periplasmic expression of carbonic anhydrase in Escherichia coli:a new biocatalyst for CO2 hydration[J]. Biotechnol. Bioeng., 2013, 110:1865-1873.
[27] FACRE N, AHMAD Y, PIERRE A. Biomaterials obtained by gelation of silica precursor with CO2 saturated water containing a carbonic anhydrase enzyme[J]. J. Sol-Gel. Sci. Technol., 2011, 58:442-451.
[28] FAVER N, CHRIST M L, PIERRE A C. Biocatalytic capture of CO2 with carbonic anhydrase and its transformation to solid carbonate[J]. J. Mol. Catal. B:Enzyme, 2009, 60:163-170.
[29] PANDA S S, PANDA B P, MOHANTY S, et al. Synthesis and properties of castor oil-based waterborne polyurethane cloisite 30B nanocomposite coatings[J]. J. Coat. Technol. Res., 2017, 14(2):377-394.
[30] PARK J M, KIM M, LEE H J, et al. Enhancing the production of Rhodobacter sphaeroides-derived physiologically active substances using carbonic anhydrase-immobilized electrospun nano-fibers[J]. Biomacromolecules, 2012, 13:3780-3786.

[1] TANG Quan, GUO Yanglong, ZHAN Wangcheng, GUO Yun, WANG Li, WANG Yunsong. Catalytic combustion of propane over PdxPty-ZSM-5/Cordierite monolithic catalyst [J]. CIESC Journal, 2019, 70(3): 944-950.
[2] XIA Hongtao, ZOU Siyu, XIAO Jie. Numerical simulation of shear-thinning droplet impacting on randomly rough surfaces [J]. CIESC Journal, 2019, 70(2): 634-645.
[3] CHEN Chen, WANG Ying, LIU Hong, CHEN Yan, YAO Mingdong, XIAO Wenhai. Exploring the key structural properties affecting the function of multi-step phytoene dehydrogenase CrtI [J]. CIESC Journal, 2019, 70(1): 189-198.
[4] DING Jiao, YIN Yaoqi, BAI Yaohui, ZHOU Xiangyang, LIU Qihai, YIN Guoqiang. Fabrication and performance of NiO-BZCYYb anode-supported solid oxide fuel cells (SOFCs) by in-situ dip coating technique [J]. CIESC Journal, 2018, 69(S1): 136-142.
[5] HUANG Jin, LI Xiaopeng, WANG Ting, HU Yanxin, SHENG Xinxin. Heat transfer performance of copper surface treatment MWCNTs/PA based on composites [J]. CIESC Journal, 2018, 69(7): 2956-2963.
[6] LI Shuangxi, SUN Xiandong, ZHANG Peng. Analysis of influencing factors of sealing face performance of mechanical seal coating [J]. CIESC Journal, 2018, 69(7): 3181-3189.
[7] ZHANG Liang, LIU Xiaochen, LIU Guiyan, LÜ Bo, FENG Xudong, LI Chun. Energy drive and regeneration in biotransformation [J]. CIESC Journal, 2018, 69(7): 2807-2814.
[8] GONG Yuan, ZHOU Jiabei, ZHU Jiahua, LUO An'an, XUE Xiao, TIAN Jian. Influence of calcium/carbonate ratio on reaction-crystallization of CaSO4-CaCO3-(NH4)2CO3-H2O system [J]. CIESC Journal, 2018, 69(6): 2533-2539.
[9] TAO Jun, ZHANG Jijun, MAO Xianhe, ZHAO Jianwei, ZHANG Dongliang, CAI Xinan. Thermodynamics analysis of in-container self-propagating high-temperature synthesis immobilization process using numerical simulation [J]. CIESC Journal, 2018, 69(5): 1846-1853.
[10] XIA Dahai, SONG Shizhe, WANG Jihui. Chaos analysis of electrochemical noise and its application in localized corrosion detection [J]. CIESC Journal, 2018, 69(4): 1569-1577.
[11] CHEN Xi, WANG Jianfang, QIAN Feiyue, GAO Junjun, SHEN Yaoliang, ZHOU Feng. Start-up of high-performance CANON reactor by alternating continuous flow and sequencing batch operation modes [J]. CIESC Journal, 2018, 69(4): 1695-1702.
[12] YIN Chunhua, MA Yewei, ZHAO Zhimin, ZHANG Haiyang, YAN Hai. Optimized preparation and characterization of novel layered cross-linked enzyme aggregates of Candida sp. lipase [J]. CIESC Journal, 2018, 69(12): 5192-5198.
[13] ZONG Xuncheng, HU Die, KAN Tingting, WANG Tingting, WU Minchen. Single site mutation of Phaseolus vulgaris epoxide hydrolase improving its enantioselectivity [J]. CIESC Journal, 2018, 69(10): 4335-4341.
[14] LIU Hu, LI Chun. Oligomeric structure of enzyme and its catalysis stability [J]. CIESC Journal, 2018, 69(1): 352-362.
[15] WANG Zhenhua, PENG Daichong, SUN Kening. Research progress of separator materials for lithium ion batteries [J]. CIESC Journal, 2018, 69(1): 282-294.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!