CIESC Journal ›› 2018, Vol. 69 ›› Issue (8): 3577-3584.doi: 10.11949/j.issn.0438-1157.20180054

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Bioactive coating prepared by bio-3D printing of castor oil-based waterborne polyurethane mixed with carbonic anhydrase

CUI Jiandong1,2, CUI Zhaohui1,2, SU Zhiguo2, ZHENG Chunyang3, MA Guanghui2, ZHANG Songping2   

  1. 1 College of Bioscience and Bioengineering, Hebei University of Science and Technology, Shijiazhuang 050018, Hebei, China;
    2 State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China;
    3 Beijing Organoid Bio-Tech Co., Ltd., Beijing 100192, China
  • Received:2018-01-15 Revised:2018-04-02 Online:2018-08-05 Published:2018-04-13
  • Supported by:

    supported by the National Natural Science Foundation of China (21676276, 21676069, 91534126).


Castor oil-based anionic waterborne polyurethane (WPU) mixed with carbonic anhydrase (CA) was used to prepare bioactive coatings by bio-3D printing technique and regular coating methods. Interactions between CA and WPU were studied by dynamic light scattering, IR, SEM-EDC, TGA, and contact angle analyses. The results showed that the CA could attach tightly to WPU nanoparticles through the electrostatic interactions with anionic groups on the polyurethane. The activity recovery of bioactive coatings prepared by bio-3D printing technology was 4-fold higher than that of the conventional coating method, reaching 50.51%. The higher activity recovery could be attributed to the thin layer, smooth surface, and uniform distribution of CA in the 3D printed coating.

Key words: bio-3D printing, castor oil based waterborne polyurethane, coating, enzyme, immobilization, biocatalysis

CLC Number: 

  • O643.3

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