CIESC Journal ›› 2020, Vol. 71 ›› Issue (10): 4520-4531.doi: 10.11949/0438-1157.20200214

• Reviews and monographs • Previous Articles     Next Articles

Application of synthetic biology in manufacture of bio-based plastics

Yanqin XU1(),Xizhi YANG1,Ruoshi LUO1,Yuhong HUANG2,Feng HUO2,Dan WANG1()   

  1. 1.National-Municipal Joint Engineering Laboratory for Chemical Process Intensification and Reaction, School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400044, China
    2.Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China
  • Received:2020-03-02 Revised:2020-04-25 Online:2020-10-05 Published:2020-05-21
  • Contact: Dan WANG E-mail:xuyanqin666@163.com;dwang@cqu.edu.cn

Abstract:

Synthetic biology is a new discipline that uses engineering ideas as a guide to transform and reconstruct natural biological genomes, synthesize new biological components, construct new metabolic routes, and produce novel products or obtain new phenotypes. Bio-based plastics are plastics produced under the action of microorganisms or the chemical reactions using natural materials as raw materials. The usage of synthetic biology to construct engineered strains to produce bio-based plastics has become a hot topic in academia and industry. This paper reviews the development of synthetic biology and important techniques in the field of synthetic biology, focusing on the research progress in the field of metabolic pathways and engineering optimization for the construction of bio-based plastic polymer monomers and derivatives such as polyhydroxyalkanoate, nylon, polylactic acid, and butylene glycol succinate using synthetic biological techniques.

Key words: synthetic biology, bio-based plastics, metabolic engineering, nylon, engineering strains

CLC Number: 

  • TK 6

Table 1

Examples of applications of engineered bacteria or engineered cells to produce bio-based plastics"

工程细菌或工程细胞改造方式/手段原料产物单体/生物基材料应用文献
Escherichia coli丙酰辅酶A转移酶(pct)以及真养产碱杆菌的PHA合成酶等基因葡萄糖3HVPHA[29]
Escherichia coli大肠杆菌中导入固氮菌属的phaABC基因和phaP基因甘油PHBPHB[30]
烟草烟草中导入含有不动杆菌属和巨大芽孢杆菌基因CO2PHBPHB[31]
Escherichia coli敲除基因消除代谢途径;过表达赖氨酸脱羧酶基因葡萄糖戊二胺尼龙5,4和尼龙5,6等[32]
Corynebacterium glutamicum大肠杆菌中的木糖异构酶基因xylA、木酮糖激酶基因xylB与赖氨酸脱羧酶基因cadA在谷氨酸棒状杆菌中共表达木糖戊二胺尼龙5,10和尼龙5,12等[33]
Corynebacterium glutamicum培养基中添加吐温40葡萄糖戊二胺尼龙5,4和尼龙5,12等[34]
Escherichia coli基因DavBDavA过表达酪氨酸5-氨基戊酸尼龙5和尼龙6,5等[35]
Escherichia coli基因LeuALeuBLeuCLeuDKivDPadA过表达酪氨酸6-氨基己酸尼龙6[36]
Escherichia coli乙酸激酶基因ackA敲除,过表达乳酸脱氢酶基因LDH葡萄糖PLAPLA[37]
Escherichia coli导入丙酮酸羧化酶基因pyc葡萄糖丁二酸PBS[38]

Fig.1

Applied fields of synthetic biology"

Fig.2

CRISPR/cas9 gene editing technology"

Fig.3

Application market distribution of bio-based plastics in 2017"

Fig.4

Regression cycle multienzyme molecular machine of 6ACA[36]"

Fig. 5

Various routes of 5AVA biosynthesis from L-lysine in microorganisms[79]"

Fig.6

Gene ackA knockout principle[37]"

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