化工学报 ›› 2020, Vol. 71 ›› Issue (5): 2248-2255.DOI: 10.11949/0438-1157.20191375

• 生物化学工程与技术 • 上一篇    下一篇

纳米氧化锌的生物法合成及固定脂肪酶的研究

尹春华(),彭思雨,马垒珍,张海洋,闫海   

  1. 北京科技大学化学与生物工程学院,北京 100083
  • 收稿日期:2019-11-13 修回日期:2020-01-13 出版日期:2020-05-05 发布日期:2020-05-05
  • 通讯作者: 尹春华
  • 作者简介:尹春华(1972—),博士,副教授,chyin@ustb.edu.cn
  • 基金资助:
    国家自然科学基金项目(21606016)

Biosynthesis of ZnO nanoparticles and their application in lipase immobilization

Chunhua YIN(),Siyu PENG,Leizhen MA,Haiyang ZHANG,Hai YAN   

  1. School of Chemical and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China
  • Received:2019-11-13 Revised:2020-01-13 Online:2020-05-05 Published:2020-05-05
  • Contact: Chunhua YIN

摘要:

采用植物乳杆菌合成了纳米氧化锌粒子并成功用于脂肪酶的固定化。将筛选得到的高耐硫酸锌的植物乳杆菌株LP4用于纳米氧化锌的合成,采用扫描电镜、透射电镜和X射线衍射等一系列分析测试手段对得到的产物进行了表征。结果显示合成的材料为直径9~35 nm球形颗粒,在359 nm处有最大吸收峰,晶体呈六方体纤锌矿结构,X射线衍射峰与标准纳米氧化锌对比结果一致,这些结果表明植物乳杆菌株LP4成功合成了纳米氧化锌。然后将合成得到的纳米氧化锌粒子用于固定假丝酵母(Candida sp.)脂肪酶,与普通氧化锌以及传统法合成的纳米氧化锌粒子相比,生物法合成的纳米氧化锌固定效果最好,固定化酶的酶活收率分别比普通氧化锌和传统纳米氧化锌提高114.2%和20.5%。论文还对该生物纳米固定化酶的pH和热稳定性以及重复使用性能进行了测定,结果表明酶固定化后稳定性明显提高,而且具有较好的重复使用性能。

关键词: 纳米粒子, 脂肪酶, 固定化, 生物合成, 氧化锌

Abstract:

Zinc oxide nanoparticles (ZnO-NPs) were synthesized by a strain of L. plantarum and used as carriers for the immobilization of Candida sp. lipase. Firstly, a strain of L. plantarum with high tolerance to zinc sulfate was screened and used to synthesize ZnO-NPs. The synthesized particles were characterized by SEM, TEM and XRD and UV-vis spectroscopy tests. The ZnO-NPs are found to be almost spherical in shape, with a radius of 9—35 nm. XRD analysis indicated the biosynthesized particles had a hexagonal wurtzite structure and the corresponding X-ray diffraction peaks were consistent with the standard ZnO-NPs. UV-vis spectroscopy revealed that the biosynthesized ZnO-NPs displayed a clear absorption peak at 359 nm. These obtained results proved that ZnO-NPs were successfully synthesized. Furthermore, the biosynthesized ZnO-NPs, native ZnO and traditional ZnO-NPs were used as carriers for the immobilization of Candida sp. lipase, respectively. The results showed that the biosynthesized ZnO-NPs were the most suitable for the lipase immobilization, and the recovered activity was 114.2% and 20.5% higher than that of the native ZnO and traditional ZnO-NPs, respectively. The pH and thermal stabilities of the lipase immobilized on the biosynthesized ZnO-NPs showed a considerable increase in stability compared to the free lipase. Moreover, the immobilized lipase showed good reusability.

Key words: nanoparticles, lipase, immobilization, biosynthesis, ZnO

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